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Stromal vascular fraction cells were isolated following established protocols [14].
Mayor Vincent C. Gray today said the District's public-safety officials are responding to the bombings at today's Boston Marathon by following established protocols designed to heighten sensitivity to potential risks in the wake of apparent terrorist attacks in other parts of the country.
ChIP was carried out following established protocols.
The pyrosequencing was performed following established protocols [21].
Genomic DNA was extracted from peripheral blood leukocytes following established protocols.
Immunofluorescence and Western Blotting were performed following established protocols [18], [43].
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Qualified semen ejaculates (>1 mL volume, >60% motility; >0.5 billion/mL concentration) were diluted with tris-citric acid extender containing 0.0 (control), 5.0, 10.0 and 20.0 ng/mL ALA at 37 °C and cryopreserved following established protocol.
Plasma cortisol levels were determined by radioimmunoassay following established protocol [13].
We used human epithelial cell line, HeLa to propagate C. muridarum following established protocol [30].
For immunofluorescence labeling, each experimental and control group consisted of DSPP labeling following established protocol.
Growth hormone (GH) content in embryo and juvenile, as well as plasma GH levels were determined by enzyme linked immunosorbent assay (ELISA) following established protocol [44].
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