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Following energization with succinate, mitochondria underwent a small and transient depolarization upon the addition of Ca2 +, which is the expected response to Ca2 + accumulation (Fig. 1 A).
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A red shift of Rhodamine 123 absorbance and fluorescence occurred following mitochondrial energization.
Mitochondrial transmembrane was estimated as the difference in fluorescence signals following substrate energization and complete depolarization and collapse of the membrane potential following addition of uncoupler.
At the end of each experiment, the maximum fluorescence signal (Fmax) was monitored after complete de-energization of the mitochondria following addition of CCCP (6 µ m) and antimycin A (0.5 µ m).
Therefore the endogenous energization-dependent activation of proton conductance following addition of succinate requires a high membrane potential.
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Adiabatic compression of the magnetospheric magnetic field, ΔB, results in a particle energization and flux enhancement, ΔJ, that follows as ΔJ/J ~ ΔB/B.
The uptake of DASPMI in mitochondria follows Nernst relations (9) and allows for straightforward determination of the mitochondrial energization state (7, 8).
Nonthermal Electron Energization from Magnetic Reconnection in Laser-Driven Plasmas.
Generally, Controlled Switching Device (CSD) is used during de-energization as well as energization operations of shunt reactors.
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