The lesion was performed using a Hamilton syringe at the following coordinates: AP: −2.9 mm; ML: +1.3 mm; DV: −4.6 mm.
The following coordinates were used: anteroposterior +10.2 mm; lateral −0.8 mm; dorsoventral −6.0 mm [72].
The two anterior infusions were made at the following coordinates: 1.3 mm posterior to bregma, ±1 mm lateral to bregma, 2 mm ventral from skull surface.
The tips were placed at the following coordinates with respect to bregma: 1.0 mm posterior, 6.5 mm lateral, 6.5 mm ventral.
The two posterior DH infusions were made at the following coordinates: 2.1 mm posterior to bregma, ±1.5 mm lateral to bregma, 2 mm ventral from skull surface.
Using a stereotaxic frame (Kopf Instruments, Tujunga, CA), the guide cannula was fixed in the hippocampal brain region according to the following coordinates: L: 1.2 mm; R: −1.8 mm; P: −2.0 mm (Paxinos and Franklin stereotaxic Atlas, 1997).
The viral solution was stereotaxically injected into the primary motor area of the right hemisphere with the following coordinates: anteroposterior from bregma 0.33 mm, lateral from bregma 1.58 mm and ventral 0.5 mm.
Injection of malonate (pH 7.4) was performed into the left striatum via a cannula inserted at the following coordinates relative to bregma: AP: 0.5 mm, Lat: 3.5 mm, V: 6 mm from the skull (Paxinos & Watsons' atlas).
The two EEG electrodes were secured with dental cement at the following coordinates: anterior/posterior from bregma (AP) 1.5 mm, lateral (ML) 1.5 mm and AP −3.5 mm, ML −3 mm.
All the animals were implanted with double guide cannulae (26-gauge; Plastics One Roanoke, VA, USA) located just above both OBs at the following coordinates with respect to the bregma: AP, +5 mm; ML, ±1.5 mm; DV, −1 mm.
In each case, a single 2 µl injection of viral vector was delivered to the SN at a rate of 0.2 µl/min according to the following coordinates from bregma: AP, −5.2 mm; ML, −2.0 mm; DV, −7.5 mm (from skull at bregma) using a microinjector (Stoelting, Kiel, WI) and according to the atlas of Paxinos and Watson [39].
