Exact(59)
The 2,404 miRNA sequences were aligned using ClustalX [ 43] with the following alignment parameters: gap opening, 22.50; gap extension, 0.83; and bootstrap value, 100.
Sequence variants were detected by visual examination of sequences following alignment using Consed version 19.0 (090206) [19.0
Sequence variants were detected by visual examination of sequences following alignment using Consed version 19.0 [ 19].
One hundred and fifty-three unique haplotypes were recovered following alignment.
(B ) Percent identity of the different domains in SNX9 and SNX18 following alignment with ClustalW.
Reads from RNA sequencing that remained unmapped following alignment were assembled using Trinity version r20140717 [ 35].
The filtering process is used to efficiently eliminate most of the unrelated reads to speed up the following alignment process.
The GS Amplicon Variant Analyzer software identified a total of 1714 variants following alignment of the raw sequence data.
Reads from DNA sequencing that remained unmapped following alignment to the reference genome were assembled using MaSuRCA 2.3.2 [ 34].
The Greengenes 16S rRNA gene database was used for 16S rRNA phylogenetic analysis with the following alignment parameters: >97% identity, minimal alignment 40 bp.
Similar(1)
In this work, we predict the TM-score using the following alignment-dependent features: sequence identity, distribution of various per-position scores such as mutation score, solvent accessibility score, secondary-structure similarity score and distribution of gap sizes.
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