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This mutant could not add a mannose residue to dolichol-PP-GlcNAc2, as indicated by a loss of ALG1 activity and secretion of N-GlcNAc2-modifed exoglucanase following a temperature shift from 25 to 37°C.
Western blot detection using a M2-Flag antibody that recognizes the FSH-tag of each LT fusion protein revealed uniquely sized proteins with expected molecular weights (Figure 1b). Figure 1c shows the robust expression observed in homozygous transgenic (F2) Thyroid Receptor-β (TRβ; NR1A2) embryos at successive time points following a temperature shift to 37°C.
Here, we characterized the protein expression of A. fumigatus following a temperature shift from 30°C to 48°C.
The translational efficiency of individual mRNAs at 25°C and following a temperature shift to 37°C (after 30 min or 60 min) was defined as the ratio of the hybridization signal in fraction-W over that of fraction-U, using a 2-fold change between conditions as the cut-off value for a change in translational efficiency.
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ibpA gene, encoding for the small bacterial heat shock protein IbpA, undergoes high level of induction following a temperature up shift and has been associated with oxidative stress response in E. coli [12], [13].
Here, we show that in the southern North Sea the proportion of decapods to bivalves doubled following a temperature-driven, abrupt ecosystem shift during the 1980s.
For example, only one experiment that involved a temperature shift from 37°C. to 25°C.
This complex was shown to form spontaneously in cell extracts subjected to a temperature shift.
The unspliced form of SUS1 mRNA (intron retention) accumulates after a temperature shift.
Cortical polarity developed as a function of time following the temperature shift.
First, we considered all mRNAs that shifted from fraction-U to fraction-W following the temperature shift to have a temperature-induced increase in translational efficiency (two-fold cutoff).
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CEO of Professional Science Editing for Scientists @ prosciediting.com