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The analyses of RNA primary and secondary 5′ ends followed previously established protocols [ 30] with the following modifications.
Surface ruptures generally followed previously documented active fault traces, except in the northeastern and southwestern sections of the Futagawa segment.
Surface ruptures generally followed previously documented active fault traces, except in the northeastern and southwestern sections of the Futagawa segment. 5.
Whereas, VCD caused a reduction in expression of AMH (d4 d8), it followed previously reported initial disruptions in Kit signaling induced by VCD (d2).
In contrast, rainfall in China followed previously wet conditions, resulting in additional flooding, although less damage than expected; there was about $85 million in crop and fishery damage in Zhejiang.
Fluorescent labeling followed previously described protocols [25].
Experiments followed previously published procedures [3], [11], [12], [13], [14].
PCR reactions and sequencing followed previously published protocols [13], [25].
Counting larval hemocytes followed previously published protocols [97], [98].
Surveys for predated rats followed previously described methods [20].
The glycan purification procedure followed previously described method [24].
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