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Full length sequences of GmGT-2A and GmGT-2B were obtained by PCR with the same primers as used in followed localization experiments.
To support the idea of peroxisome proliferation during phagocytosis, we followed localization of the YFP KNIESKL fusion protein during infection of macrophages.
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The ability to follow localization of an open form of Dia allowed us to separate the roles of Rho1 in Dia activation and localization.
To address these questions, we first had to establish an assay to follow localization of Cdx2 mRNA in living mouse embryos.
Following localization and stabilization of the lesion with one hand, a hollow bore needle (22G, 23G, or 25G) was inserted directly into the mass and the needle was moved swiftly in and out for approximately 10 seconds.
Following localization of these five proteins to the site of new centriole formation, recruitment of microtubules completes the assembly process, giving rise to a ninefold- symmetric centriole ∼100 nm in diameter (Pelletier et al., 2006).
Following localization of the TMS site on the participant's scalp outside the scanner using Brainsight neuronavigation (see above), the participant was placed in the scanner and the TMS coil carefully fixed against the marked site on the head using a custom coil holder.
However, RACK1 localization follows the localization pattern of iMELK suggesting that in the epithelial cells it may specifically interact with iMELK at the tight junctions.
To identify the element required for localization of Cdx2 mRNA, we first generated a series of truncated forms of the Cdx2 gene and followed their localization.
Since hemocytes are adherent and can be visualized through the cuticle by fluorescence microscopy, we followed their localization in vivo after CrPV injection.
We also followed the localization of a Pkc1-GFP fusion protein that consists of the entire Pkc1p fused through its C-terminus to a green fluorescent protein.
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