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Recommended flow rate and backpressure specifications were followed (flow rate: 1 mL/min, maximum backpressure: 0.3 MPa).
Other techniques, such as cell surface staining using recombinant MHC/HLA class I or II tetramers followed flow cytometry analysis, enumerate the number of antigen (epitope -specific CD8+ or CD4+ T-cepitope -specificy.
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On the basis of ridge max analysis, the optimum conditions for synthesis were as follows: flow rate 0.1 mL/min, temperature 51.5°C and substrate molar ratio 1 4.14.
The happiness that follows flow is of our own making, and it leads to increasing complexity and growth in consciousness".
Csikzentmihalyi asserted that "the happiness that follows flow is of its own making, and it leads to increasing complexity and growth in consciousness," which is the whole point.
LC MS conditions were as follows: flow rate: 100 μL/min; injection volume: 5 μL, column temperature: 45 °C.
The settings are as follows: flow rate of carrier gas (helium), 2 ml/min; source temperature, 200°C; interface temperature, 250°C.
The conditions for the chromatography were as follows: flow rate 1.0 mL/minute, UV detector wavelength 235 nm, injection volume 50 μL, and column temperature 25°C.
The other chromatographic parameters were as follows: flow rate 0.4 mL/min, temperature 35°C, run time 35 min, post time 12 min, and DAD detection range 200 400 nm.
Following flow cytometric analysis, plantlets were hardened and transferred to pots in greenhouse (same conditions as for donor plants) following the procedure described by Prem et al. [ 29].
Chromatographic conditions were as follows: flow rate of 5.0 mL of He/min, column temperature of 35 °C, and injector and detector temperatures of 120 and 150 °C, respectively.
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