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The results summarized in Table 8 indicate that top classifications are obtained by the Code 13 in seven organisms; followed by the Code 17 with four top classifications (with the Code 13 ranks 2nd in 2 cases and the Code 10 ranks 2nd in 1 case, each of these 3 cases is shown in Table 8 on the row following that of the top performer); and the Code 6 with one top classification.

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We designed a cassette consisting of a splice acceptor followed by the coding sequences for Green Fluorescent Protein (GFP) and a polyadenylation site which was inserted in a reverse orientation in self-inactivating lentiviral vectors.

Interviews 4 6 were then coded using the final list of codes, which were followed by the coding of interviews 7 9.

This was followed by the coding of transcripts and memos made during participant observation in each case.

It is followed by the coding region of Pontellina plumata GFP (copGFP) [12] and an unpaired splice donor site.

The differences are largest for the beginning of the 3'UTR as well as the end of the 5'UTR followed by the coding region.

For both FFPET and frozen samples, the highest proportion of annotated reads mapped to the 3' untranslated region (50% of annotated FFPET reads and 41% of annotated frozen reads), followed by the coding exon (14% FFPET and 22% frozen).

A 2.5 kb linear DNA fragment containing the chloramphenicol resistance cassette (CmR) followed by the coding region of the rpoN gene was constructed using cross-over PCR [ 65].

To test the importance of this motif, we constructed a reporter containing 2.5 kb of the Pfk promoter, followed by the coding sequence for GFP.

We made reporter constructs containing different lengths of the promoter region of Ldh, followed by the coding sequence for green fluorescent protein (GFP).

This fragment was combined using fusion PCR with a cassette containing a (Gly-Ala 5 Gly-Ala 5linker by the coding region ofollowednd the A. fumigatus pyrG gene, such that the C-terminus of Vps32 was fused in frame to the (Gly-Ala)5 linker followed by the mRFP coding region (De Sofza et al., 2004; Yang et al., 2004).

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