Sentence examples for followed by adding a from inspiring English sources

The B16F1 cells were incubated with non-radioactive FEBZA (10−11 M 10−4 M) at room temp for 1 h followed by adding a fixed amount of 4-[18F]FEBZA to each well.

The composite electrodes were prepared by dispersing the PPy/MWCNT composites or carbon nanotubes, pure PPy samples and PTFE (5%), followed by adding a small amount of ethanol and NMP to yield a homogenous paste.

For a typical ELISA process, a capture anti-cTnI antibody against cTnI is firstly immobilized onto the surface of plastic well, followed by adding a patient sample containing cTnI for binding the capture anti-cTnI antibody.

In brief, thioglycolic acid was added into the mixed precursor solutions of Cd(CH3COO 2 2H2O and Zn(CH3COO 2 2H2O, which was adjusted to pH = 9 by using the solution of 2 mol/L sodium hydroxide, followed by adding a freshly prepared oxygen-free NaHTe solution with vigorous stirring under nitrogen atmosphere.

Then, take 30 ml anhydrous ethanol and 0.17 g cetyltrimethyl ammonium bromide (CTAB) blended by sonication for 10 min, then add 30 ml above graphite oxide aqueous solution and vigorous stirring to obtained mixture, followed by adding a specific amount tetraethoxysilane (TEOS) and magnetic stirring 10 min, finally the ammonium hydroxide was used to adjust PH to 10, then continually stirring 2 h.

To prepare the growth solution, 5 mL of 0.01 M HAuCl4 and 0.35 mL of 10 mM AgNO3 were mixed with 95 mL of 0.1 M CTAB, followed by adding a freshly prepared 0.55 mL of 0.1 M AA solution to reduce the Au3+ to Au0.

Fe2O3-promoted ZrO2 was prepared by the coprecipitation method using a mixed aqueous solution of zirconium oxychloride and iron nitrate solution followed by adding an aqueous ammonia solution.

This was followed by adding an aqueous phase (0.6 ml of water containing 5 mg/ml PVA).

The volume was adjusted to 600 µl by water followed by adding an equal volume of phenol, pH 4.2.

The released dsDNA was then end repaired, followed by adding an 'A' base at the ends.

RSEM estimate values were multiplied by 10 followed by adding an offset of 10 and subsequently log2 transformed.