The inhibition of the interaction between the fluorescent BH3 peptide and Bcl-2 or Bcl-XL is followed by measuring a decrease in fluorescence polarization.
ArcLight displayed a different response from Case12, with an initial small peak of fluorescence directly after eATP addition followed by a rapid decrease in fluorescence and gradual increase to approach the baseline (Fig. 4, ArcLight).
As shown in Fig. 2A this manoeuvre induced in the control cells an increase in fluorescence followed by a decrease, suggesting the presence of functional chloride channels on the plasma membrane.
Other variants exhibit a transient increase in fluorescence during the 10 ms phase followed by a decrease during the rate-limiting phase.
Inhibition of HDAC activity was monitored by a decrease in fluorescence signal.
Evidence of fluorescence quenching (indicated by a decrease in fluorescence upon addition of the arrestin mutant) would indicate proximity between the Trp and fluorophore.
The rate of GB release was followed by the decrease in fluorescence at 450 nm.
Unwinding traces show a lag phase, followed by an increase in fluorescence during unwinding, and then a final phase with slower fluorescence increase.
In the presence of RepD, there was a lag phase, followed by a rapid increase in fluorescence.
Hypertension was followed by a gradual decrease in SAP.
