All HKII proteins were tagged with green fluorescent protein (GFP) in order to follow their expression and localization by fluorescence microscopy.
To ensure that transduced cells were expressing gene product derived from the transduced cDNAs, we first considered the possibility of adding a tag to the transduced cDNAs to follow their expression and translation into protein.
We have demonstrated here that a high-throughput real-time luciferase-based screening system is valuable for tagging novel promoters and genes in banana and to follow their expression pattern throughout in vitro development and LT treatment.
Moreover, we followed their expression profiles at the level of pri-miRNAs, pre-miRNAs, and mature miRNAs in the following developmental stages: 1-, 2-, 3-, and 6-week-old, and 68-day-old plants.
Following their expressions and body language, imagine a silly conversation that they could be having.
When you start talking to somebody mimic slightly their body image if their tense then tense your posture slightly, follow their facial expressions,.
We allow each other that freedom, we can follow their body language, the expression on their face, where they're going with music because we know each other so well.
To distinguish potentially conserved clusters of co-expressed genes, we selected the key pluripotency-associated factors Pou5f1/ POU5F1, Sox2/ SOX2 and Nanog/ NANOG and followed their temporal expression dynamics (Fig. 1B).
Following their co-expression in the developing skeleton, the interaction between RUNX2 and VDR became involved in further definition of the features of the mammalian skeleton (Shen and Christakos 2005, Lian et al. 2006).
Following their exposure to γIR, expression of endogenous p21 was monitored over a period of 10 h in the absence and presence of doxycyclin.
With global investigations of miRNAs in subjects with ID, followed by their expression analysis, our understanding of the role of miRNA in ID pathogenesis will be further improved.
