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We followed the established method for generalized NCA, using a regularization factor of 0.8 to regulate the strength matrix S [ 13].
Following the established methods [32], antler tip tissue for mRNA extraction was rapidly separated into five morphologically defined layers (Fig. 4) under a dissecting microscope, snap frozen in liquid nitrogen and stored at −80°C.
We follow an established method of dealing with the problem of only one treated state by using the remaining untreated states to conduct a permutation test in which we construct the empirical distribution of β01 by estimating β ̂ 01 for each of the control states, treating each control state as the treated state (see Abadie et al. (2010) and Buchmueller et al. (2011))12.
FISH followed the established protocol [ 16, 56].
Following a previously established method, reactive groups were generated on electrospun PCL fiber surfaces via base-catalyzed hydrolysis of the ester bond in the PCL backbone followed by conversion of the resulting carboxylates to carboxylic acid groups with hydrochloric acid (Hartman et al. 2010).
Following a previously established method [52], hair segments (ca 20 mg) were weighed in a glass tube and incubated in 1 ml of 1 M NaOH at 95°C for 15 minutes in the presence of stanozolol D3 as an internal standard (I.S).
The H. pylori conserved core families (CGFs) and pan-genome families (PGFs) were estimated followed by previously established method [ 19, 22].
The identification of fusions in IMG follows well established methods [4].
Following previously established methods [ 14], we inoculated 10 mL of LB media with a loop taken from a freezer stock of Arabinose minus E. coli (REL1206), and the culture was grown overnight at 37.0 °C.
The staining procedures followed established methods [34].
Reconstruction and model development of the metabolic network followed established methods [ 5, 3].
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