Many of them follow restrictions on interaction with nonrelatives, and the head coverings most of them wear make it impossible to blend in.
Banks that once took over entire floors of Manhattan nightclubs and reserved Midtown steakhouses are continuing to follow restrictions on the office holiday party, many of which have been in place since the 2008 financial crisis.
The proposed merger of Time Warner and Charter Communications is set for approval by government regulators, who want the combined company to follow restrictions designed to prevent it from stifling the growth of streaming video services.
Under laws passed in the aftermath of a sweeping investigation into the CIA's brutal interrogations of suspected terrorists during the administration of George W. Bush, the U.S. must follow restrictions on interrogations laid out in the Army Field Manual, which prohibits torture.
The sanctions follow restrictions imposed by the US on Venezuela, including a travel ban on top officials from the Latin American nation who are accused of human rights violations.
Following restriction endonuclease digestion of intact Tetrahymena Mt DNA, the terminal restriction fragments were slightly "smeared" due to the variable length of the telomeres.
Following restriction endonuclease digestion, DNA samples were electrophoresed through 0.7% agarose gels, and transferred to Nytran SuPerCharge membrane (Schleicher and Schuell) according to standard procedures [72].
Following restriction digestion with XhoI and PacI, these APP, tau, SOD1 and TXN 5' UTR sequences were inserted between the XhoI and PacI sites of php15 to generate pGL4.15cmvAPP5, pGL4.15cmvtau5, pGL4.15cmvSOD1.5 and pGL4.15cmvTXN5 respectively.
Following restriction and adaptor ligation, there followed two successive rounds of PCR amplification.
