Sentence examples for follow medium from inspiring English sources

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Following medium change, pretreated PC12 cells were incubated with 10 nM rrC5a (30 min at 37°C) and analyzed with the Annexin V apoptosis detection kit for Annexin V and Propidium Iodine as described above.

Following medium aspiration, INS-1E cells were lysed directly in culture dishes by adding TRIzol reagent (Invitrogen, Carlsbad, CA, USA).

Following medium refreshment, plates were exposed to 1 min constant excitation at 470 nm using a Zeiss inverted fluorescence microscope.

Cells were fixed 4 h, 3 days and 5 days after seeding as follows: medium was removed, 100  μl 10% TCA in PBS was added to each well and left for 60 min at 4 °C.

Cells were split once confluent as follows: medium was removed and the monolayer washed with PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4).

All measurements were performed in triplicate as follows: medium was replaced with 50 μl CyQuant solution (Life Technologies, Paisley, UK) prepared according to manufacturer's protocol and cells were incubated for 1 hr at 37°C.

Following medium removal, 150 μl of DMSO were added to each well and plates were gently shaken for 10 min. Optical absorbance was determined at 570 nm with a Spectra Max M5 (Molecular Devices, USA).

Following mediums were prepared by changing C or N sources in CM while keeping other contents constant.

We have interviewed 1382 patients with the SF-36 questionnaire during the follow up (medium 1291+/− 166 days).

Cells were cultured in serum containing (2%) Ep-Wang medium [ 16] for 24 hours followed by medium exchange to serum free medium (Ep-sfm) [ 15].