To relate molecular bond formation at the cell surface to cell triggering, molecular binding and the cell response must be followed in parallel within the same time scale.
In order to determine which cell type(s) represented the growth factor target(s), we followed in parallel cultures from unselected PBMC and from pure populations of preselected CD34+ cells.
These possibilities can only be tested more systematically when a straightforward identification of male and female Squalius embryos becomes possible and the expression patterns can be followed in parallel in both sexes.
A low-risk control group, composed of children that have no family history of autism, is typically followed in parallel.
We focused on carriage density and duration in experimental carriers, but also compared both parameters in natural carriers, who were followed in parallel.
To explore specificity against cognate isoleucine in tRNA-dependent pretransfer editing by IleRS, the initial rates of AMP and Ile-tRNAIle formation were followed in parallel to extract the kAMP/ kIle-tRNA ratio.
To accomplish this, the initial rates of AMP formation [ kAMP (Table 1)] and tRNA misacylation [ kVal-tRNA (Table 1)] were followed in parallel assays relying on [α-P]ATP and [P]tRNA, respectively.
Metabolites were separated using reversed phase liquid chromatography (LC) followed, in parallel, by high-resolution nanofractionation onto 96-well plates to assess the bioaffinity of parent compounds and metabolites and accurate mass MS and MS analysis for structural identification of formed metabolites.
