Following behavioural testing, rats were sacrificed for Golgi-Cox analysis.
Following behavioural analysis (see below) spinal segments were collected from sham operated and spinal cord injury animals at 24 hours, 4 days, 4- and 10-weeks after surgery (n = 6 at each time).
Following behavioural analysis, mice were perfused with 4% (w/v) paraformaldehyde.
Following behavioural testing, all rats received a lethal overdose of sodium pentobarbitone (60 mg/kg; Euthatal, Rhone Merieux, UK) and were then transcardially perfused with 0.1 m phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 m PBS (PFA).
Following behavioural training, each monkey was implanted under general anaesthesia and aseptic conditions with a headpiece (to allow head fixation) and a recording chamber placed over the central sulcus (Lemon, 1984; Baker et al., 1999).
Following behavioural testing, the SHR rats as well as age-matched Wistar Kyoto rats (WKY/NHsd) [ 37] were stunned and rapidly decapitated at PND 55 60 (the WKY/NHsd were used as additional reference strain in the biochemical analyses).
Following behavioural testing, the animals were administered an intraperitoneal injection of a lethal overdose of Euthatal (200 mg/mL sodium pentobarbital, Marial Animal Health Ltd., Harlow, Essex, UK) and perfused intracardially with 0.1 m phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 m PBS (PFA).
Methane emission rates tended to follow this behavioural pattern with peak emission rates corresponding to peaks in eating activity.
The population state-pair distribution is more complex than if foraging together is advantageous: Fig. 2b shows that the distribution of state-pairs tends to follow the behavioural thresholds mentioned above, lending a rectangular shape to the distribution.
The majority of these treatments are recommended by the European Guidelines for the Management of Chronic Low Back Pain[ 4], where the options considered to have the most benefit for patients are exercise combined with education programs and exercise programs that follow cognitive behavioural therapy principles.
