The unique feature of this approach is that the curvature description is sufficient for protein folding without any long-distance interactions other than the excluded-volume interactions.
Numerous choices have to be made in setting the conditions for successful folding/unfolding, and here a major challenge remains to identify conditions that lead to completely reversible folding without any hysteresis.
Both a shorter (residues 77 124) and a longer (residues 65 124) prosegment catalyze plasmepsin II folding at rates more than four orders of magnitude faster compared to folding without prosegment.
Manipulation of cellular chaperones could also shift the equilibrium towards increased folding, without the drawback of channel inhibition.
The nascent protein can then undergo folding without interference from other constituents of the intracellular environment [ 30].
The hinge purposes to separate the two components of the protein fusion, thus allowing each to undergo folding without steric hindrance from the other [ 22].
These data suggest that Hsp90 selectivity might buffer protein folding; without Hsp90, intrinsically unstable proteins would have been even less stable and expressed at a lower level.
These compounds bind to specific positions in domain V of the large ribosomal RNA and prevent its activity in protein folding, without affecting its function in protein synthesis (Tribouillard-Tanvier et al, 2008b).
