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The origami product was loaded into the gel electrophoresis wells after completing the folding phase immediately.
A higher concentration of carbon and smaller grain size resulted in an 18% increase in energy absorption in a secondary folding phase cycle.
The folding phase diagrams of SH3 as a function of denaturant concentration ([C]), mechanical force (f), and temperature exhibit an apparent two-state behavior, without revealing the presence of the elusive N⁎ states.
During the first cycle of the secondary folding phase, observed differences on an average value basis were less than or equal to 3% for maximum loads, 6% for minimum loads, and 3% for energy absorption.
Studies of the folding pathway of large proteins whose kinetics is complicated due to the formation of several intermediate states are most frequently impeded or totally impossible because of rapid folding phase occurring during instrument dead time.
Crush characteristics were evaluated and, for each material type, observed differences were less than 7% for maximum and minimum load magnitudes and less than 2% for energy absorption, displacement, and mean load quantities in both the initial phase and the secondary folding phase cycles.
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The Internal Albanides composed of the Mirdita and Korabi zones, which are characterized by the presence of allochthonous ophiolites (Upper Jurassic) and by many folding phases (Upper Jurassic, Eocene etc).
This clearly illustrates a folding of phase space around the limit-cycle, and is further portrayed in the video in Additional file 3. We now show how this can be understood using phase-amplitude coordinates.
Moreover, residues identified from native structure as connector hubs and which had been shown to be those that were protected during the transition phase of folding were indeed more stable (less flexible) well beyond the transition state.
In fact, the rate constant of the fast phase of folding, monitored at 215 nm, was 1.65 (± 0.8) s-1 for both proteins.
However, gas phase folding of proteins that function in far less polar environments such as membranes might be more similar to their folding in solution, a topic which we plan to address in future studies.
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