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Our observations validate the force-clamp technique as a powerful approach to determining the free-energy landscape of proteins collapsing and folding from extended states.
Comparing the number of bits set in the short and long version of ECFP4 and ECFP6 (data not shown) revealed that the number of collisions introduced by folding from 1024 bits to 16384 bits was very low.
Mutations that disrupt peripheral tertiary contacts give large and nearly uniform increases in re-folding of M, whereas the same mutations give at most modest increases in folding from Itrap.
Such studies can reveal determinants of the folding mechanism beyond the gross topology, and can dissect the residues required for folding from those required for stability or function.
uLCDD-01 and uMM-01 have the lowest values/most favorable free energy of folding from the thermal denaturation data (ΔG(4°C)) while both AL proteins show the highest/less favorable values (Table II).
MD, including the longest simulations performed, has been extensively used for ab initio protein structure prediction, 132– 136 aiming to simulate protein folding from scratch, although this is not the preferred strategy to obtain theoretical model of protein structure.
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Oldsmobile offered a video player with a flat screen that folded from the roof, and they soon sprouted everywhere.
Most origami is folded from square paper and from a single sheet.
Structures were folded from extended structures by BP method.
Origami techniques are used to develop crawling robots that self-fold from flat-pack designs.
This approach identified and distinguished genes such as "folD" or "FolD" from the word "fold".
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