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But the characteristics needed for proteins amenable to this kind of study, such as folding capability, long-term stability, and high solubility, require robust and expeditious methods for the identification and optimization of target protein domains.
These differences in folding capability might explain different phenotypic outcomes even among siblings sharing a given phenotype [ 2], and also possibly, the different response to pyridoxine supplementation among PH1 patients with the same phenotype [ 63, 64].
We achieved ultimate flexibility including bending and folding capability of the hybrid structures in Figure 3 [40].
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It may additionally guide the design of novel mutants with impaired conducting or folding capabilities as a tool for selectively knocking down or knocking out connexin function.
Since the re-folding capability after heat-induced denaturation was previously correlated to higher performance during recombinant expression, a unique heating step can be envisaged to screen constructs that can provide high yields of correctly-folded proteins.
The capability of non-folding proteins and regions to interact with collections of partners is utilized in organizing complex protein-protein interaction networks.
Effects of folding technique application to architecture configuration capabilities are presented.
Other exciting developments in our experimental capabilities for measuring protein folding reactions include the use of NMR relaxation measurements such as off-resonance N rotating frame [ 31] or relaxation dispersion [ 14] methods, which are capable of detecting non-native species when their population is exceedingly low (down to ∼0.5%).
GFP was chosen for this assay because it can be detected by fluorescence without antibodies, and also because its auto-fluorescence capability relies on its correct folding, thus allowing us to easily monitor the proper folding of the inclusion-associated tagged GFP.
This osteointegrable implant was designed with biomechanical capabilities using a unique "Greek wave" folding pattern.
Although simplified models have the capability of catching nontrivial aspects of the folding problem, the approximations involved are not really suitable [ 5].
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