Sentence examples for folded snare from inspiring English sources

Based on the CD spectrum, we estimated that about 50% of the amino acids in the protein are in an alpha-helical configuration, consistent with a fully folded SNARE four-helix bundle in the chimeric protein.

This model also established the structure and the extension of the folded SNARE complex h as a function of the contour length l.

The purified chimeric SNARE protein was eluted from the column (Superdex 200 10/300 GL) in one major peak corresponding to a molecular weight of 57 kDa, consistent with a folded SNARE four-helix bundle.

DOI: http://dx.doi.org/10.7554/eLife.03348.005 10.7554/eLiFigure48.006 Figure 1 figure supplement 3. The chimeric neuronal SNARE protein folds into a homogenous SNARE four-helix bundle with an expected molecular weight.

The folding energy of the structured part of the SNARE complex as a function of the contour length gives the folding energy landscape of SNARE folding.

Longin domains have been suggested to regulate trans-SNARE complex formation, possibly by folding over their SNARE helix (Gonzalez et al., 2001; Mancias and Goldberg, 2007; Martinez-Arca et al., 2000, 2003b; Tochio et al., 2001).

Li et al. (2014) have recently shown that the association of the N-terminal half of VAMP2 to the N-terminal t-SNARE triggers folding of the t-SNARE C-terminal domain.

The energy and position of the transition state is important for characterizing the energy landscape of SNARE folding described later in the text.

We adopted a simplified energy landscape model to derive the energy and rate of SNARE folding at zero force ('Materials and methods') (Gao et al., 2012; Xi et al., 2012).

Therefore, analogous to a car engine, an efficient SNARE engine is expected to change gears to meet increasing resistance as SNAREs fold towards membranes.

We propose that the step-wise assembly enables reversible folding of SNARE complexes, as shown in our calculations.