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It has a native-like backbone topology and shows some local deviations from the native structure, revealing that the structure of the folded region of an early folding intermediate can be as well defined as the native structure.
A Leu213Pro substitution in VIPAR is predicted to result in the disruption of this alpha-helix in the core of a folded region of VIPAR structure, and might affect the overall folding of the protein and its interactions with VPS33B and/or other potential binding partners.
The folded region exhibits only one dark line, suggesting monolayer graphene structure (Fig. 2b) [19].
The first folded region contained a putative regulator of G-protein signaling (RGS) domain.
The structure shows that the folded region of the intermediate is much larger than the proton slow-exchange core at 25 °C.
The second folded region contained a highly protected PKA binding site and a more solvent-accessible PDZ binding motif, which may serve as a potential targeting domain for D-AKAP2.
Similar(44)
Similar folded regions were detected by limited proteolysis.
To confirm the exact number of layers in the sample, we observed their folded regions by using an electron beam parallel to them.
One can see that the surface of the nanowall is covered with some nanoprotrusions and folded regions and that the thickness is not uniform.
Deuterium on-exchange revealed two regions of low deuterium exchange that were surrounded by regions of high exchange, suggestive of two distinctly folded regions, flanked by disordered or solvent accessible regions.
The SAED patterns (Figure3d,e) of the folded regions of the middle part (areas D and E) show that they are polycrystalline with textured structures, which contain a lot of dislocations and stacking faults.
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