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"We still fold time.
"If you were to fold time and space you'd always recognize a teenager's room," says editor and film writer Luke Goodsell.
A total of 28 miRNAs (Fold time ≥1.5; P ≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.
Hmgcr which is the rate limiting enzyme in cholesterol biosynthesis was repressed 2-fold after 12 h of TSA treatment and showed increasing down regulation over 24 h (4 fold) and 48 h (5.3 fold) time points.
Genes involved in lipid and fatty acid metabolism such as ApoA5 and Acat2 were found to be maximally down regulated at 12 h (12 fold) and 24 h (16 fold) time points respectively while ApoL1 was down regulated (11 fold) at 12, 24 and 48 h time points.
Levels of Cyp27A1 or sterol 27-hydroxylase which participates in the conversion of cholesterol to bile acids was also found to be initially down regulated at 6 h (1.8 fold) and increasingly over the 12 (8.8 fold) and 24 h (10.3 fold) time points.
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99% selected genes showed 1.2-2 fold times change.
Of the 17 down-regulated miRNAs, 14 miRNAs had a fold change of more than two fold times.
FLLL-11 and FLLL-12 are respectively, 223 and 298 fold times more toxic in colorectal cancer cells than in WI-38 normal human lung fibroblasts compared to GO-Y030 which is only 14 fold times more toxic.
In particular, IIGP1 was found to be highly up-regulated in our microarray data (90 fold times).
Of the 11 miRNAs that were up-regulated in the tumor tissue, 8 miRNAs had a fold change more than two fold times.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com