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The greater expression of pro-apoptotic gene Bim regulated by NF-κB-IkB and FOXO1 is supported by fold regulations calculated for various apoptotic and pro-inflammatory biomarkers carried out by RT-PCR.
For data analysis, threshold cycle values (Ct values) were obtained and fold regulations (up or down) of the test group were calculated over the control group using 2−ΔΔ C t algorithm.
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Table 2 shows the top 33 of these dual regulated genes, arranged by their fold regulation through MPA compared with the relevant natural hormone in each cell line.
The p values and fold regulation levels were calculated from six experiments Up-regulated genes shown in bold.
Any gene is considered as regulated by TGF-β if qRT-PCR results showed > two fold regulation as compared to untreated cells.
We also analyzed the distribution of the fold regulation under these conditions as displayed in Fig. 2B.
The fold regulation threshold was 3. (b) Gene expression of Setdb2 in whole brains at 8 h and 24 h post LPS administration.
The fold regulation threshold was 2. (b e) The expression levels of Cav1 (b), Gjb1 (c), Gjc2 (d), and Cdh1 (e) in whole brains at 8 h and 24 h post LPS administration were analysed.
Genes with greater than 2 fold regulation were confirmed by qRT-PCR.
Fold regulation shown in all tables is the average ratio of median value for each gene.
Figure 4 shows MA plots comparing normalised Log2 expression levels with the fold regulation, 1hr−0hrs (Figure 4a), 4hrs−0hrs (Figure 4b), 1hr+GMCSF−1hr (Figure 4c) and 4hrs+GMCSF−4hrs (Figure 4d).
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