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Table 2 shows the top 33 of these dual regulated genes, arranged by their fold regulation through MPA compared with the relevant natural hormone in each cell line.
The p values and fold regulation levels were calculated from six experiments Up-regulated genes shown in bold.
We also analyzed the distribution of the fold regulation under these conditions as displayed in Fig. 2B.
The fold regulation threshold was 3. (b) Gene expression of Setdb2 in whole brains at 8 h and 24 h post LPS administration.
The fold regulation threshold was 2. (b e) The expression levels of Cav1 (b), Gjb1 (c), Gjc2 (d), and Cdh1 (e) in whole brains at 8 h and 24 h post LPS administration were analysed.
For calculations and statistical analysis, fold changes were calculated using (2−ΔΔCT) method in the experimental samples.61 Statistical analysis for gene expression was performed using the one sample t-test, which estimates the calculated difference (in fold regulation) between experimental and control samples.
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Gene amplitude rankings were comparable between the datasets: out of the top 347 regulated genes in the DCE/RNA-seq dataset (with >1.84-fold regulation), ∼80% were corroborated by the starve-synch/microarray dataset (Fig. 3, left).
A similar switch design based on miR-122-T afforded 7-fold regulation when placed in tandem, indicating that this approach can be extended to additional miR-T.
Optimized on switches introduced into adeno-associated virus (AAV) vectors afforded 10-fold regulation of two antiviral proteins in AAV-transduced cells.
We achieve up to 10,000-fold range in constitutive gene expression and 100-fold regulation of gene expression with inducible promoters and use these parts to record DNA-encoded memory in the genome.
RT-qPCR results of selected genes ranged from 1.5-fold to 36-fold regulation (Figure 6A), whereas microarray results ranged from 1.5-fold to 10-fold regulation for the same genes (Figure 6B).
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