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Reactivity of RG7787 with sera from immunotoxin-treated patients was >1000 fold reduced.
Better absorption was confirmed by reduction in lipid profile with several fold reduced dose in mouse model.
Overall, PRLR expression was 2.4 fold reduced in adenomas and 4.8 fold reduced in adenocarcinomas compared to normal tissues.
The apparent dissociation constant determined was about 20 fold reduced (Fig 5F).
On the other hand, Proteobacteria were shown to be 1.8 fold reduced after exercise training (p < 0.05) (Additional file 5C).
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Surprisingly, the affinity was only ~3-fold reduced between ssDNA and RNA (Fig. 1c and Supplementary Fig. 2).
Due to ablation of the native adenovirus tropism, the infectivity of this AdV was at least 35-fold reduced on 293 cells.
By in vitro mutagenesis, it was shown that a mutation at codon D588N of UL54 conferred 9-fold reduced susceptibility to PFA, while a mutation at codon V781I induced 4-fold reduced susceptibility to PFA and GCV.
All engineered variants triggered potent ADCC with up to 100-fold reduced EC50 values compared to non-engineered variants.
Mediator release assays using rat basophil leukemia cells showed that these variants displayed a 1 × 105-fold reduced allergenic potency as compared to wild-type protein.
The human umbilical vein contractility assay responded to L-alanyl-histamine (EC50 54.7 μM), but the APN inhibitor amastatin massively (17-fold) reduced its apparent potency.
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