Sentence examples for fold pooling from inspiring English sources

Individual DNA concentrations were normalized to 40 ng/ul and then used to prepare 8 fold pooling plates for TILLING screens.

Screening of genomic DNA from the TILLING library was conducted on two fold pools in consideration of the high mutation density associated with this hexaploid wheat EMS-mutagenised population.

For screening the mutant library, M2 DNAs were first subjected to a four-fold pooling, resulting in 752 pooled samples contained in eight 96-well microtiter plates.

DNA was then pooled using a one-dimensional eight-fold pooling strategy.

HRM analysis of EMS-mutagenized maize was conducted with 5-fold pooling [ 10].

An 8-fold pooling strategy was employed in the current study.

With 4-fold pooling all of the mutant bands were detected, as they were with the 8-fold pools, whereas with 12-fold pooling only a subset of the mutant bands were seen on the TILLING gel.

A simple one-dimensional eight-fold pooling strategy was employed using a Xiril liquid handling robot (Xiril AG, Hombrechtikon, Switzerland).

In this test, we detected only three of the five mutations that we had discovered by 4-fold pooling.

Lowering the pooling fold (for example, 4-fold pooling) may increase the sensitivity of mutation detection in sorghum.

For TILLING assays, heteroduplexes are derived from the melting and re-annealing of wild type and mutant amplicons, generated by two-fold pooling of genomic samples before PCR.