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GPx activity was increased in both liver (1.7 fold, P < 0.01) and erythrocytes (3.5 fold, P < 0.05).
In PBMCs, the qPCR platform readily detected SEB-induced cytokine expression above background (IFNγ, 148.8 fold, p = 0.001; IL-2, 130.9 fold, p = 0.008; TNFα, 7.4 fold, p = 0.001; Wilcoxon Signed Ranks test; Figure 4).
Deletion of agr resulted in diminished levels of the lukF-PV transcript, compared with 923, at 3 (10 fold, p<0.001) and 6 hours (1.8 fold, p = 0.02).
(d) Pfmrp2, appears to significantly induce the less MQ sensitivity clones 3D7 (1.32 fold, p = 0.003) and FCB (1.46 fold, p = 0.023) compared with W2 (0.96 fold).
The known cAMP-responsive genes encoding p27/Kip1 (1.54 fold, p = 0.009) and Atf3 (1.65 fold, p = 0.012) also scored positive albeit below the 2-fold cut-off.
Glutamate decarboxylase (GadA), up 7.2 fold, p = 0.021.
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Treated wounds featured thicker granulation tissues (1.7-fold, P < 0.0001) enriched in collagens (1.3-fold, P < 0.0104).
Methionine synthase expression was significantly decreased in dwarf compared to wild type (0.48-fold, p = 0.023).
Results: Cortisol levels were higher in maternal plasma than fetal (4-fold, P <.mob031).
Significant increases in Cu/Zn SOD mRNA levels (6.0-fold, P <.005) and catalase mRNA (8.0-fold, P <.005) and its protein levels (2.3-fold, P <.005) and lipid peroxidation levels (5.3-fold, P <.005) were observed at the lowest concentration (10 μmol/L) of α-tocopherol treatments.
Compared to wild type, the Ames dwarf had increased expression of methionine adenosyltransferase 1a (2.3-fold, p = 0.013), glycine N-methyltransferase (3.8-fold, p = 0.023), betaine homocysteine methyltransferase (5.5-fold, p = 0.0006), S-adenosylhomocysteine hydrolase (3.8-fold, p = 0.0005), and cystathionase (2.6-fold; tended to be increased, p = 0.055).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com