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Furthermore, inhibition of mitochondrial respiration by rotenone (20 nmol/L) greatly abolished Hcy-increased IFN-γ secretion from 80.71 ± 15.10 pg/mL in control cells to 40.81 ± 18.16 pg/mL in rotenone-treated cells (Fig. 3F), and cell proliferation from 1.15 ± 0.03 to 0.51 ± 0.03 fold of control (OD450/OD630 absorbance) in rotenone-treated cells (Fig. 3G).
Hcy-activated IFN-γ secretion and cell proliferation were also significantly blocked with nocodazole (10 μmol/L) pretreatment, from 164.8 ± 14.36 to 49.27 ± 13.68 pg/mL for IFN-γ secretion and fold of control (OD450/OD630 absorbance) from 1.64 ± 0.01 to 0.07 ± 0.05 for cell proliferation (Fig. 5I and 5J).
Two subunits of C1 complex, C1R and C1QL1, were showing expression ∼0.7 fold of control.
Other notable genes down-regulated by trypsin were cathepsin D (0.3 fold of control) and telomerase reverse transcriptase (0.5 fold of control), while protein phosphatase 2C was upregulated (3 fold) by PAR2 activating peptide.
Also there were 6 genes that were 3 fold or more down-regulated versus normal (e.g. 0.3 fold of control).
Stimulation with SB431542 alone did not significantly alter RANKL levels in the culture supernatant (0.04±0.07 fold of control).
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Among the studied non-conventional carbon sources i.e., soya flour 40 g/L and sesame oil 30 mL/L were found producing 1109.37 mg/L (1.24-fold of control) and 1196.75 mg/L (1.34-fold of control) lipstatin respectively.
HHcy significantly increased Rhod-2 positive T cells to a 2.3-fold of control cells, from 3.67% ± 0.7% in control T cells vs. 8.46% ± 0.3% in HHcy treated cells (Fig. 1B).
Confluent monolayers of bovine aortic endothelial cells (BAECs) were subjected to a shear stress of 12 dyn/cm2 over intervals ranging from 0.5 to 30 min. Shear stress increased Cbl phosphorylation to 2.9-fold of control and Cbl association with the regulatory PI-3 kinase subunit p85 to 5.4-fold.
As shown in Fig. 1A, total cellularity increased in a dose-dependent fashion up to four-fold of control values.
1D and 1E, increased doses of either drug resulted into an enhanced cell death in MO-SC transfected cells for up to 6.7- and 4.9-fold of control in IDA and ETO treatments, respectively.
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