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By using an optimized production protocol, VLP titers were increased 2.4-fold obtaining 2.8 μg of Gag-GFP/mL or 2.7 × 109 VLPs/mL according to ELISA and nanoparticle tracking quantification analyses, respectively.

The model has a conserved folding module in ZNFs, which is the most probable folding obtained.

Onset of induction was fast, being detectable within 60 min, with maximal induction (45-fold) obtained after 16 h.

Under hypoxic conditions enhancements of 5-fold, 20-fold and 60-fold were obtained with CDDP and 1, 0.1 and 0.01 microM mitomycin C, respectively.

Fold change obtained by qRT-PCR correlated well with the direction of fold change obtained from the normalized intensity data, confirming the validity of the microarray gene expression patterns (Table S6).

The ratios between paired samples (fold change) determined by qRT-PCR were, however, greater than the fold change obtained for the same gene by microarray analysis.

The compression of fold changes obtained by RNA array was more apparent for genes with high fold changes such as Cte1 and Decr1 (Fig. 2).

Very similar results with 7 9 fold increase in the presence of ionophore and the final contrast of 13 15 fold were obtained for Case16.

The fold changes obtained from these two reaction levels are also very similar (Data S3).

The data reported for each gene represents the median fold ratio obtained from at least three slides.

The comparison of gene expression fold change, obtained both by microarray analysis and qRT-PCR, was assessed by Pearson correlation.