We have recently identified a number of Quantitative Trait Loci (QTL) contributing to the 2-fold muscle weight difference between the LG/J and SM/J mouse strains and refined their confidence intervals.
Into these folds muscle buds migrated from the ventral border of the adjoining myotomes, giving rise to radial muscles which motivated the fins and were the forerunners of the intrinsic muscles of the hand (Bunnell).
In this study, the steady-state level of acox1 mRNA increased 3-fold in liver, 3-fold in intestine, 2-fold in muscle and 2.5-fold in heart of zebrafish fed ≥ 0.50% clofibrate compared to zebrafish fed < 0.50% clofibrate.
Zebrafish acox1 hnRNA increased 2.5-fold in liver, 2-fold in intestine, 8-fold in muscle and 4-fold in heart of fish fed ≥ 0.50% clofibrate compared to zebrafish fed < 0.50% clofibrate indicating an increase of transcriptional initiation of the acox1 gene in these tissues.
Proteasome activity was significantly (P < 0.01) increased 2.3 fold in muscles from control, suspended muscle as compared to muscles isolated from weight bearing control mice.
The expression level of Smprx6 in the ink sac was defined as 1, and expression was 460.71-fold higher in the pancreas, followed by liver (148.76-fold), gill (90.15-fold 90.15-fold6.6-fovary testis (11.53-fold), muscle (7.10-fold), and stomach (1.01-fold).
Muscle samples were first diluted 60-fold in PBS, subsequent dilutions and the calibration curves were done in 60-fold control muscle in PBS.
The steady-state level of fabp1a hnRNA increased 6-fold in intestine of zebrafish fed ≥ 0.50% clofibrate and > 5-fold in muscle of zebrafish fed 0.50% clofibrate.
fabp7a hnRNA increased > 3-fold in liver of zebrafish fed 0.50% clofibrate and 7-fold in intestine of zebrafish fed ≥ 0.75% clofibrate, while fabp7b hnRNA increased 6-fold in muscle of zebrafish fed ≥ 0.50% clofibrate compared to control zebrafish.
In this study, the steady-state level of fabp1a mRNA increased 1.5-fold in the intestine of zebrafish fed 0.50% and 0.75% clofibrate and increased 4-fold in muscle of zebrafish fed 0.50% clofibrate compared to zebrafish not fed clofibrate.
