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In case of HEK293 cells the results of the colony-forming assays performed are presented as fold increase in relation to pEPI-1 [CMV-IEP]- [EGFP-IRES-BSD]-ΔMARS.
Since this vector did not result in any stably selected NIH3T3 cells, the results of the colony-forming assays in NIH3T3 cells are presented as fold increase in relation to pEPI-1 [hCMV/EF1P]- [EGFP-IRES-BSD]-ΔMARS,.
For normalization, we used 18S RNA [GenBank accession no. NT_039649.7 (National Center for Biotechnology Information 2008)] as the reference gene to derive the fold increase in relation to the sham control group.
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Values are given as -fold increase in relation to the level in commercially purchased "normal" sera.
The signal intensity was measured for each band on an immunoblot, normalized to the loading control, and the fold increase was determined in relation to the appropriate DMSO treated control.
With reference to alcohol consumption, the risk increased in relation to number of drinks, with a six-fold increase for ⩾8 drinks/day.
Comparison of the yield showed that the production rate of solution-blowing was increased 30-fold in relation to electrospinning.
Of the 2,910 differentially-regulated putative drought-responsive transcripts detected, 1,671 were up-regulated (57.4%), with the most responsive transcript (Affymetrix probe-set ID Os.53488.1.S1_at; GenBank AK072922, 'expressed protein'), increasing 15.7-fold in relation to the control.
Similarly, fukutin MO-injected embryos show a significant up-regulation of lama1 (∼2.3-fold), lamb1 (∼2.1-fold) and lamc1 (∼1.5-fold) in relation to control embryos (Fig. 5A C).
A similar behavior was shown for BNBD4 mRNA expression, except at 1 mM, in this case we observed an increase of ∼2-fold in relation to bMEC only infected).
Expression ratios are displayed as fold change in relation to the wild-type control samples.
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