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PD-1-/ also showed a mild elevation, whereas PLPtg/PD-1-/ double mutant mice exhibited a robust upregulation of CD8+ T-cells in optic nerve sections (approximately 35-fold increase compared to wildtype mice and a more than 8 fold increase in comparison to PLPtg mice, Figure 1A).
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Data are shown as fold increases in comparison to the level of hemoglobin in vehicle-treated cells.
Analysed by flow cytometry, the fluorescence signal of living HEK293 cells expressing the codon optimized variant was 5 8 fold increased in comparison to the predecessor with unaltered codon usage but identical amino acid sequence (data not shown).
The model was experimentally validated, yielding a lipase production of 2283.70 ± 118.36 U/mL which represents a 6.7-fold increase in comparison to the non-optimized medium.
Other studies have shown raised LDL levels with time spent on high fat diet [42 44], with approximately a two-fold increase in comparison to chow-fed animals.
This means a statistically significant 2.4-fold increase, in comparison to the average number of microcarcinomas diagnosed over the study period.
The highest TA was detected in the female floral bud sample, but this level of expression represents only a one-fold increase in comparison to the average TA in all organs.
In contrast, a K d ser of 4.7 mM was obtained for mutant G385F, a ~4-fold increase in comparison to the wild-type SPT, suggesting that this mutation has had some impact on both PLP and substrate binding.
We observed that treatment of H37Rv-infected DCs with 10 mM NAC and 20 μM L-GSH resulted in 2.5-fold increase in the expression of CD80 compared to H37Rv-infected DCs and 5-fold increase in comparison to uninfected control category.
The sequential CENP-A IP demonstrated a 3-fold enrichment of ectopic CENP-A nucleosomes compared to the centromeric fraction in SW480 cells, which constituted a 10-fold increase in comparison to HeLa, wherein ectopic CENP-A is depleted with regard to the centromeric fraction (3.02 versus 0.32 enrichment for SW480 and HeLa, respectively).
Consistent with our observations on the CD80 marker, we also found a similar trend with the expression of CD86 marker; that is, treatment of H37Rv-infected DCs with 10 mM NAC and 20 μM L-GSH resulted in 2.5-fold increase in the expression of CD86 compared to H37Rv-infected DCs and 5-fold increase in comparison to uninfected control.
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