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The first, composed of unamplified 454 and 10ng Illumina metagenomes, contains intermediate levels of duplication (14.6 to 42.7%) and few high-frequency (>10 fold) duplicate reads (0.06 to 5.1%).
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Adjusted data were further filtered to remove genes with opposite ratio values and genes differing more than 3 fold in duplicate analysis of the same NK sample.
Transported to the world, her wiles inspire The same frustration in rich connoisseurs Who pay the price for nourishing the stem To keep the bloom fresh, as if their desire To live forever lived again through hers: But in a day she fades, though every fold Be duplicated in fine shades of gold.
Expression of 379 genes increased in Zap1TC-expressing cells an average of ≥ 1.5-fold in duplicate arrays.
Discrepancies of more than 3-fold between duplicate assays were presumed to result from technical error, and the assay was repeated.
When comparing expression in cells grown in LZM + 3 μM ZnCl2 vs. LZM + 3 mM ZnCl2, 182 genes were up-regulated ≥ 1.5-fold in duplicate arrays under this very low zinc condition.
Chromosomes X and Y have approximately three- and 17-fold more duplicates, respectively, than expected under an assumption of equal duplication frequencies across all chromosomes.
The larger nature of somatic replicons (when averaged) correlates with the fact that somatic cells require ∼10-fold longer to duplicate their genetic content relative to the embryonic system.
After blocking with 200 µl/well of 3% BSA in PBS-T, 60 µg/ml of each of six competitor/soluble antigens (AMA1 from strains FVO, HB3, 3D7 or CAMP, a mixture of the 4 allelesleles designated NM, and DiCo mix designated DM) were titrated 3-fold over 9 duplicate wells at a 50 µl/well final volume.
Each compound was measured in 16-point, 2-fold dilution in duplicate.
Rhodanese homology domain is an alpha beta fold domain found duplicated in the rhodanese protein.
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