Quantification of total tumor fluorescence at the 24 hour time point indicated an overall 10 12 fold brighter probe signal for the ABP compared to the substrate (Fig. 3B).
He sits, arms casually folded, bright eyes darting from student to student before he picks on one on the front row.
Therefore, to maximize our chances to observe a transfer event, we constructed a new probe where super-folder GFP (sfGFP), a fast folding bright variant of GFP (Pédelacq et al., 2006), is fused to the type II signal sequence (OMsfGFP).
In a direct comparison of the brightness characteristics between the new proteins and EGFP [22], which is the most widely used genetically encoded fluorescent marker and a typical reference point for brightness comparisons, the new proteins turned out to be 2.2-fold brighter overall, since both their ME and QY are higher (Fig. 2B, C).
The fluorescence intensity is ≈5- and ≈9-fold brighter from Rose Bengal (RB) and Pt II) octaethylporphine (PtOEP), respectively, on silver colloid-deposited paper as compared to uncoated paper.
The red-emitting PLR3 was ∼2.5-fold brighter than CBR in living HEK293T and HeLa cells, an improvement consistent with the importance of the Km value in low ATP environments.
Compared with existing organic dyes and fluorescent proteins, nanotech probes can offer signals that are several folds brighter and hundreds of times more stable [5, 6].
Interestingly, the fluorescent intensity of pHluorin(M153R) was approximately 2.5-fold brighter than that of pHluorin.
Moreover, mRuby is about ten-fold brighter compared to EGFP when being targeted to the endoplasmic reticulum.
They possess high molar extinction coefficients (79,000 M−1 cm−) and quantum yields (0.92), which make them more than two-fold brighter than the most common FP marker, EGFP.
