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Isoelectric focusing was run on Protean IEF System (Bio-Rad) for 60,000 V-Hrs.
The isoelectric focusing was run on IPG strips with a non-linear pH range of 3-10 and a length of 7 cm (Bio-Rad) using the ZOOM® IPGRunner™system from Invitrogen.
Isoelectric focusing was run using the Protean IEF Cell Apparatus (Bio-Rad) with the following voltage/time gradient: 100 V for 1 minute, 250 V for 30 minutes, 4 000 V for 2 hours, and 4 000 V for 10 000 V-hr (Vhour).
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While isoelectric focusing is running, cast the 2D slab gels.
She worries about her wayward son, of course, but her main focus is running the kingdom with her ill-gotten husband, Claudius, played compellingly by Nigel Gore as a sort of bureaucratic variation on one of the dapper gangsters the Kray Brothers.
RaiseAChild's main, up-front focus is running media campaigns, which include streetlight banners and public transportation advertisements, "to encourage the LGBT community to build families through fostering and adoption".
Decide on a running shoe if your primary exercise focus is running, either on a treadmill or outdoors.
Isoelectric focusing (IEF) was run following a stepwise incremental voltage program on an IPGphor Isoelectric Focusing System (GE Healthcare Life Sciences, Taipei, Taiwan).
Isoelectric focusing (IEF) was run in immobilized pH-gradient gel strips (IPG strips 180 mm×3 mm; Pharmacia, Freiburg) with a pH range of 3 to 10 [49], [49].
After loading the labeled samples, isoelectric focusing (IEF) was run following the protocol provided by Amersham BioSciences.
Isoelectric focusing (IEF) was run on an Ettan IPG phor II (GE Healthcare, CA, USA) using 24 cm nonlinear immobilized pH gradient strips (pH 3 10; GE Healthcare).
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