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Cells were stained for α-tubulin and the degree of spindle pole focusing was determined.
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The origin of the radioactive focus was determined to be the renal pelvis.
The quantitative focus was determined considering the time spent in conducting the reuse process.
In a separate experiment, cells were exposed to BnDTPA-F3 (2 μM) at increasing specific activities (0 to 6 MBq/μg or 0 to 20.6 MBq/nmol) for 2 h, and the number of γH2AX foci was determined as above.
The percentage of cells presenting HR foci was determined microscopically.
The point of best focus was determined to be 0.722 mm in front of the lens, and the depth of field was approximately 3 microns.
The frequency of micronucleated and apoptotic cells containing either Rad51 or pS2056DNA-PKcs foci was determined by analyzing at least 1000 cells per sample.
The number of mice bearing lung foci was determined.
Spacing between α-actinin foci was determined with ImageJ software.
Pictures were then merged, and the frequency of colocalization with GRSF1 foci was determined.
The signal intensity ratio (SIR) of the foci was determined as previously described.
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CEO of Professional Science Editing for Scientists @ prosciediting.com