Sentence examples for focusing strip from inspiring English sources

Equal amounts of paired LMN and CON samples, plus the reference sample, were mixed and loaded onto an isoelectric focusing strip (DryStrip, 24 cm, pH 4 7, GE Healthcare).

Fifteen microgram of each sample was loaded into each of two pH 3 10 isoelectric focusing strips (GE Healthcare, Piscataway, NJ, USA) and separated to equilibrium.

Serum samples were treated with a mixture of Affigel-Blue and protein A (5 : 1) for 1 h to remove high abundance protein (e.g. immunoglobulin and albumin) and were displayed using 11 cm, pH 4 7 isoelectric focusing strips for the first dimension and 10% acrylamide gel electrophoresis for the second dimension.

Isoelectric focusing strips were then equilibrated for 15 min in modified SDS sample buffer (50 mM Tris pH 6.8, 6 M urea, 30% glycerol, 2% SDS, 1% DTT and trace bromophenol blue), separated in the second dimension by 15% SDS PAGE, transferred to PVDF membranes and subjected to eIF5A and β-actin immunoblotting as an internal control.

After focusing, strips were equilibrated for sample buffer and then overlaid onto 4 12% SDS-PAGE.

After focusing, strips were equilibrated in 50 mM Tris (pH 6.8), 6 M urea, 2% (w/v) SDS, 30% (w/v) glycerol, and bromophenol blue, containing 20 mM DTT in the reduction step (15 min) and 25 mM iodoacetamide in the alkylation step (15 min).

The protein sample was loaded onto 17- cm, non- linear pH 3 10 IEF isoelectric focusing strips (Immobiline DryStrip, Amersham Biosciences).

Loading strips were then put on re-swelled 24-cm narrow-range isoelectric focusing strips (pH 3.4 4.7) also provided by GE Healthcare.

Where total protein contents were slightly different between gels (primarily due to variations in efficiency of sample uptake by isoelectric focusing strips), spots volumes were proportional to total volume of all the spots (data not shown).

After focusing, strips were equilibrated in Tris/HCl pH 8.8 50 mM, urea 6 M, glycerol 30%% (v/v), and SDS 2% (w/v) for 10 min in buffer containing 10 mg/ml DTT followed by incubation for 10 min in buffer containing 25 mg/ml iodacetamide.

After isoelectric focusing, strips were equilibrated by agitating for 10 minutes in 50 mM Tris-HCl, pH 8.8, 6 M urea, 30% (v/v) glycerol, 2% (w/v) sodium dodecyl sulfate (SDS), 650 mM DTT and then agitating for10 minutes in 50 mM Tris-HCl, pH 8.8, 6 M urea, 30% (v/v) glycerol, 2% (w/v) SDS, 1.27 M iodoacetamide.