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The monitoring Argon ion laser beam (488 nm, 1.2 microwatt) was focused through the microscope (Zeiss Universal) to a Gaussian radius of 0.85±0.02 mm (63× objective) or 1.36±0.04 mm (40× objective).
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The laser light was focused through the objective (×20) of an Olympus BX-41 microscope.
White light images were acquired from fresh tissue using a digital camera focused through the eyepiece and objective lens of our two-photon microscope.
The essential components of a 2PP setup are a femtosecond or short picosecond laser that is focused through a microscope objective and translational stages for moving the point of the laser within the resin on three dimensions.
The most sensitive enzyme assays use lasers focused through a microscope to observe changes in single enzyme molecules as they catalyse their reactions.
Finally, the phase modulated beam is projected through a galvanoscanner for structuring control in X and Y axes and focused through a microscope objective.
The 410 nm diode laser (<50 mW) is focused through a 40× microscope objective to a spot size of ∼5 μm.
Light from a superluminescent diode (SLD, λ = 830 nm, 50 nm bandwidth; Superlum, Russia) is focused through a 10× microscope objective (Zeiss) to a 25 μm-diameter spot on the sample.
The laser beam, with an average power ranged between 10 and 40 mW, was focused through a 10× microscope objective with a numerical aperture of 0.30.
A fluorescence microscope focusses through the silica specimen onto the contact in order to image the distribution of dyed oil.
The beam was focused through a × 60 microscope objective with a numerical aperture (NA) = 1.2 using water immersion.
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