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In contrast, in the PALM image reconstruction only fluorophores that are bound to F-actin are likely to be detected as well focused spots (with 2D-Gaussian shape) and thus represented in the image.
Under this experimental condition, only the signal from F-actin molecules are detected as focused spots; while the signal from monomeric G-actin molecules spreads to a much larger area due to fast diffusion (>8 µm2/sec [24]) and is not detected.
The phase conjugate patterns for all the focused spots are retrieved by digital reconstruction of the captured holograms.
The distance between two adjacent focused spots is chosen based on the desired resolution and the speed of the system.
In our approach, we exploit all the available modes in a very high numerical aperture (NA) multimode fiber, in order to generate and scan, optimal resolution and contrast, focused spots and eventually to capture high-detail images of stained biological samples.
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A focused spot with a diameter of 37 μm was used for sample and standard analyses.
The focused spot is measured as about 35 μm in diameter.
The laser power was approximately 0.25 mW and the focused spot size was 1 μm.
With an aid of the visible emission spectra of the focused spot, polyyne forming reaction is discussed.
The process allows the ablation, fusion and/or vaporization, and finally substrate texturing in only one step by means of a concentrated energy bean in a focused spot.
The launching position of SPPs is fixed at the end of waveguide, and the focused spot is limited to the diffraction.
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