Sentence examples for focused plane from inspiring English sources
Data was analysed using ImageJ software, by choosing the most focused plane, adjusting brightness and contrast, and after instant time concatenation.
To reduce a 3-D tiled image data set to a single focused plane, custom-written processing software automatically merges adjacent fields (i.e. mosaic-ing), and then collapses the merged focal planes using either a maximum local contrast algorithm or maximum through-plane grayscale intensity (i.e. z-projection), as is often done for confocal image sets.
For instance, a flat-top profile is obtained in the selected focusing plane, with a maximum transversal intensity variation of 2.5% over 18 mm.
However, we found the fact that scanning with full power caused bleaching of PBsomes not only in the focusing plane, but also perpendicularly, in a few-micrometer flanking areas through the cell (data not shown).
Putative electrical synapses were identified as the dendritic or axonal contacts at the same focusing plane using a microscope water lens (60× magnification, numerical aperture = 0.9; resolution along the Z-axis = 0.37 µm).
The resolution deteriorates for objects outside this focusing plane.
An optimal pinhole size of 60.64 μm was used to exclude fluorescence light emitted from out-of-focus plane above and below the focusing plane.
The illuminating lines were fixed to the height of the focusing plane of the detector.
The latter is then propagated up to the focusing plane by applying the appropriate phase changes.
The propagation of the altered spherical wave up to the focusing plane has been calculated by using the angular spectrum of plane waves approach in the scalar approximation [ 14, 15, 17].
From the point of view of optical wave propagation, the problem consists in determining the disturbances of the point spread function (PSF) observed in the focusing plane that are due to the presence of the PRP. Figure 3(a) represents the intensity distribution of the converging wave in the plane just behind the PRP.
