Focusing on significantly differentially expressed genes, it was found that superhubs and hubs in the network had a lower range of divalues, while genes that encoded peripheral proteins in the network had a higher range of divalues.
To focus on significantly similar samples, we introduced the cut-off threshold C. If the sample similarity J Sa, Sx is smaller than the cut-off threshold C, J′ Sa,Sx is set to 0. On the other hand, if J Sa,Sx is larger than C, J′ Sa,Sx = (J′ Sa,Sx − C /(1 − C), so that the range of sample-to-sample similarity J′ Sa,Sx becomes 0 to 1.
Focusing on 60 genes that were significantly different between ESC and NSC conditions (t-test, 5% false discovery rate [FDR], ≥1.5-fold different), individual cell lines clustered appropriately by their culture condition (Figure 1D).
"They realise they are being focused on and significantly changed their attitudes, changing the way they hold assemblies," said Mr Mahmood.
Historical reaction to the battle has focused on the significantly stronger British position in the encounter, with three large regular warships pitted against a hastily converted merchant vessel with a significant proportion of the crew suffering from illness.
Thus, we focused on the significantly regulated genes involved in MAP kinase signalling pathway.
Our data analysis focused on identifying significantly regulated biological processes and molecular pathways (i.e., gene functional analysis) instead of testing the differential expression of individual genes.
For further analysis, we focused on six significantly regulated genes from four different functional groups: the transcription factors FOSL1 and EGR1, the secreted proteins OPN and IGFBP3, the phosphatase DUSP4, and the membrane protein TAAL6.
Interestingly, no graph (neither in the paper nor in the appendix) shows the total number of hard events over time including those occurring between days 0 and 7. Instead, attention was focused on the significantly lower rate in the PCI group when the first 7 days were excluded (4.6 vs. 8.0 %, p = 0.04).
"What we should be focusing on -- as COP21 did -- is significantly, drastically and quickly reducing our reliance on fossil fuels and moving toward renewable forms of energy.
