SI courses focus on probing questions that are of a social nature (i.e. pertaining to social arrangements, human behavior and forms of social, political and economic organization).
Traditional Affymetrix GeneChip arrays focus on probing the coding sequences of known genes, and the probes usually only cover the annotated transcripts' 3' end, hence much information regarding new transcripts (e.g. microRNAs, anti-sense transcripts and new genes), as well as splice variants of both known and unknown transcripts, are never found [1], [2].
Future research should now focus on probing these regions with behavioural and cognitive tasks designed to elicit reward-related behaviour in combination with functional MRI.
To determine the accuracy of the mitochondrial or lysosomal localization predictions, we focused on probes that exhibited a perinuclear, punctate, "organelle" associated staining pattern characteristic of the mitochondria or lysosome-targeting compounds.
Next, because the LM-PCR products were generated mainly in the 100 1000 bp size range, we focused on probes enclosed by two MspI sites with an interval length between 100 and 1000 bp (Figure S3).
We focused on probes with correlations >0.75, of which there were between 46 and 200 probes per tissue pair.
We focused on probes that were specifically targeting the genes (i.e., probes with suffix '_at' and '_a_at') and significant correlation after correcting for multiple testing (p < 1e-5).
Because all CGH experiments were performed with Sakai as the reference strain, our analyses focused on probes targeting genes present in the Sakai genome.
According to a recently published thermodynamic model for estimating gene expression levels on oligonucleotide microarrays [ 20], it is conceivable that focusing on probes closer to the 3' end would find similar increases in the detection of low abundance transcripts.
