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Rather the two DSEs present in a single DSB appear to be kept close enough to each other, either by GamGFP or perhaps by an E. coli DNA-repair or other protein(s), that only a single focus is visible.
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Sup35-GFP in A3 and A4 cells was not very different from log phase cells with the exception that more foci were visible.
After 4 washes with PBS, 200 µl of insoluble tetramethylbenzidine solution (Sigma, USA) was added to the cell monolayers and incubated at room temperature until well defined foci were visible.
Often, only one or a few foci are visible.
RFP foci are visible in shi ts -expressing SCs but not in controls.
As expected, during mid-log (nonstress) growth conditions, several WT Edc3 foci were visible.
At stage 9 only few small mRNA foci are visible, at stage 10 the mRNAs were enriched in proximity of the DNA in two large foci (see arrows).
Small KAP1 phosphorylation foci were visible after 30 min, increasing to 1 h, but were virtually undetectable at 4 h (Fig. 1E).
As expected, RPA foci were visible only in cyclin A-positive cells that are in S or G2 phase of the cell cycle.
Bacterial infection foci are visible at concentrations of fluorescent bacteria from 10 to 10 CFU; whereas, the negative control displays only background autofluorescence.
After three days of co-cultivation with A. tumefaciens, fluorescing foci were visible at the margin of scutella of the immature embryos.
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