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The SeqA focus distribution was, however, not normal after 60 and 90 minutes (compare Figure 2A and 7A).
This result indicates that the normal SeqA focus distribution is lost soon after irradiation when most forks are stalled and then slowly reappears again when forks are repaired and replication continued.
We can estimate from the above data that this DFR-based genotyping system should be scientific sound because it correlates very well with the focus distribution of the pathogen and the conventional ecotyping system that is widely used by Chinese plague scientists for typing Y. pestis[23].
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The SeqA focus distributions were found to be essentially unchanged (Figure 3, fourth panel and Table 3).
A total of 3740 autosomal SCs from 170 pachytene cells were analysed for MLH1 foci distribution based on SC length rank (Table 3).
With both 3.5- and 6.5 Gy-TBI, γ-H2AX foci distribution differed significantly from those of the sham-treated animals up to 4 days after exposure (Fig. 2C and 2D).
These findings indicate that the different patterns of γ-focus distribution are due primarily to telomere length and not other mouse-human species differences.
These findings suggest that, in addition to differences in patterns of γ-focus distribution between early and late-generation telomerase-null mice, DNA damage pathways leading to cellular senescence also differ primarily as a consequence of different telomere lengths.
In metaphase spreads from pre-senescent G2 mouse cells, 66% of the γ-foci were on the chromatid arms and 34% were on the ends, a senescence-related γ-focus distribution pattern similar to that observed in wild-type MEFs.
Foci distribution of the homozygous eGFP-Trf1KI/KI (control) or sh-Trf1, were taken as internal controls.
In cells that were allowed to re-aggregate, new cell cell contacts formed (as indicated by the focused distribution of C-cadherin) and xMELK was found concentrated at the newly formed cell cell contacts.
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CEO of Professional Science Editing for Scientists @ prosciediting.com