Briefly, SUVs containing fluorescent lipid (OG-DHPE, Invitrogen) were made as described above and were used to generate a standard curve of OG-DHPE concentration vs fluorescence intensity on a Nikon Eclipse Ti microscope using a 20× objective focusing deep into the solution and away from the glass.
For example, a 50 pA probe, with a beam semi-convergence angle of 25 mrad that is focused 100 Å deep into the sample to form a spot of 1Å diameter, would spread over an area of ~20 Å2 at the crystal surface.
Steven Jacques et al. demonstrated that a reflectance confocal scanning laser microscopy measures the decay of reflected signal as the focus moves deeper into solutions of nanoparticles, absorbing and scattering materials, and typical reflectance standards.
The optical sectioning of the root was achieved by moving the focus position deeper into the sample in 1 μm steps, which produced z-stacks of individual pictures from the same xy-area with a penetration depth of up to 60 μm.
Only Wikia, which is free and makes money through advertising, focuses on deep dives into pop cultural niches: everything you've ever wanted to know (and then some) on topics like "Star Wars," DC Comics, Harry Potter, Xbox games and "Game of Thrones".
The move, says Facebook's Jonathan Dann, "signals a shift in how Facebook is building mobile products, with a focus on digging deep into individual platforms".
However, regardless of such possibilities, as molecular research has tended to focus us ever deeper into the nucleus, it is perhaps comforting as diabetologists and endocrinologists that 'old-fashioned' hormone communication between our favourite organs remains important during gestation as well as postnatally.
The ACTV is a bit more focused, diving far deeper into the niche that is hardcore athleticism.
The rest of my teammates focused on diving deeper into some data I generated with a Python script, and ultimately found that teams moved the ball a lot less in the playoffs, and also scored fewer points per possession.
The bow and arrow, which she took up three years ago, require a concentration and focus that send her deep into herself.
