The distribution of TssL-mCh was different again, with a mixture of foci and "patchy" fluorescence tending to the periphery of the cells.
Flow cytomtery was performed using an Attune NxT Acoustic Focusing Imager (Life Technologies) and fluorescence was measured in the BL3 channel.
Figure 1 A shows focus stackings of bright and fluorescence light overlays at high magnification.
The micrographs shown here represent focus stackings of bright and fluorescence light overlays by using software (BZ-H1RE) that selects the sharpest areas from multiple frames.
In late pre-divisional cells (90 min), the helix-like pattern was maintained, and after cell division, swarmer cells had a single focus of fluorescence and stalked cells had a helix-like fluorescence pattern.
Here the deconvolution algorithm is able to improve the contrast between the fiber in focus and the gap (no fluorescence) 200 nm below sufficiently for visualization.
Previous investigations of fluorescent-magnetic nanoparticles mainly focused on the MRI imaging and fluorescence imaging for diagnosis; however, there are few studies on the multifunctional magnetic targeting drug delivery system for diagnosis and therapy [12, 13].
The presence and location of metastatic tumor foci (green fluorescence) and monocytes/macrophages loaded with the red fluorescent microspheres in these organs was determined using an optical imaging system, Berthold NightOwl Optical Imager (Berthold Technologies, Oak Ridge, TN) loaded with Berthold Winlight 32 software (Berthold Technologies).
The presence and location of metastatic brain tumor foci (green fluorescence) and macrophages loaded with the red fluorescent microspheres was determined after resection using an optical imaging system.
The distribution of metastatic tumor foci (green fluorescence) and macrophages loaded with the red fluorescent nanospheres in the brain, lung, liver, and the heart was determined after resection using an optical imaging system.
