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However, after crossing over (in late pachytene and early diplotene), no effect of RAD54/RAD54B deficiency on the reduction of irradiation-induced foci was observed.
Indeed, an increase in the number of heterochromatin foci was observed after the induction of differentiation in mouse embryonic stem cells (ESCs) using several different methods, such as immunostaining of the heterochromatin protein HP1, DAPI staining42, and monitoring the GFP-fused methyl-CpG-binding domain43.
Similarly, an increase in Rad11-GFP foci was observed in a set2-R255G mutant background compared with wild type (Fig. 2d), suggesting a role for Set2 methyltransferase activity in preventing break-induced ssDNA formation analogously to Ku70, thus providing an explanation for the increase in HR in the absence of Set2.
After MMC, a similar proportion of cells with γ-H2AX foci was observed in control and p210-transduced cells (Figure S2).
Thus, a marked increase in DNA damage foci was observed in both proliferating and differentiating Chk1+/− erythroid progenitors during erythropoiesis, whereas WT erythroid cells did not display DNA damage foci.
An emergence of small, pyroninophilic ('tigroid') foci was observed at the second, fifth and eighth days after the proliferative stimulus.
Similar(42)
In contrast, numerous fluorescent foci were observed in the cytoplasm of ∆dnaK cells.
An increase in the number of phosphorylated γ-H2AX foci is observed after Hdac1 or Hdac2 deletion.
No such GST-P positive foci were observed in rats fed control diet.
When the tumor promoter TPA was used, a significant number of transformed foci were induced concentration-dependently, whereas only a few foci were observed in control cultures.
We tested a roadside sampling technique in a pure maritime pine forest (Pinus pinaster) of ca. 1300 ha where bark beetle outbreak foci were observed following a windstorm.
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