The automated segmentation tool includes foci in the whole body starting with the highest uptake foci to the left on the x-axis (b).
We evaluated whether direct orthotopic cell microinjection, between the mucosa and the muscularis layers of the cecal wall of nude mice, drives tumor foci to the most relevant metastatic sites observed in humans and/or improves its yield as compared with previous methods.
Collectively, our findings link Rad51 binding by the C terminus of Brca2, via effects on the rate of dissolution of Rad51 foci, to the timing of mitotic entry.
Finally, following exposure to IR, the patient's fibroblasts showed a similar number of ATM-dependent MDC1, γH2AX, 53BP1 and BRCA1 foci to the normal control, although they were noticeably less bright (Supplementary Figure 1).
In contrast, ∼50 60% of class II (medium sized buds, larger than 2 μm in length) and class III (large buds with the nucleus and pnER in the bud) cells transferred CPY* foci to the bud.
A small number (<20%) of cells with small bud size (less than 2 μm in length; classified as class I cells (Babour et al., 2010), transferred CPY* foci to the bud, while the majority of cells (∼80%) contained foci only in the mother.
